Composite
Part:BBa_K415147:Experience
Designed by: Jason Stevens Group: iGEM10_MIT (2010-10-24)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K415147
Assessing p8-Fos incorporation into phage coat
We transformed XL1-Blue cells with BBa_K415147, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-Myc antibody.
The purple band at 13 kD represents the p8-Fos fusion, and is evidence of the fusion protein's incorporation into the phage coat. We are currently looking into possible explanations for the fainter band at 15 kD.
User Reviews
UNIQ1e7d6dfe4cc4453b-partinfo-00000000-QINU UNIQ1e7d6dfe4cc4453b-partinfo-00000001-QINU